Each 25-µg total protein of mucus adjusted with PBS to 70 µl was incubated with 10 µl of mushroom tyrosinase (Sigma-Aldrich) in potassium phosphate buffer pH 6.5 (5 U/reaction), and then 20 µl of 1 mg/ml L-DOPA (Sigma-Aldrich) in buffer was added. The reactions were monitored the absorbance at A475. Distilled water was used as a negative control and kojic acid as positive control. The inhibition was calculated from the formula: Inhibition (%) = [1 − (A475 in sample/A475 in control)] × 100%.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.