For immunohistochemistry (IHC) staining, the following primary antibodies were used: α-SMA (1:500), CK 19 (1:200), PCNA (1:500), PPARγ (1:100), HMOX-1 (1:200) and Nrf2 (1:100). Tissues were immunostained as described previously [27]. Masson’s trichrome staining was performed by a ready-to-use kit (Sigma-Aldrich). To calculate the positive index of each staining, three random fields were counted by microscope (BZ-X710; Keyence). Serial sections were used for immunohistochemical staining.

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