For immunohistochemistry (IHC) staining, the following primary antibodies were used: α-SMA (1:500), CK 19 (1:200), PCNA (1:500), PPARγ (1:100), HMOX-1 (1:200) and Nrf2 (1:100). Tissues were immunostained as described previously [27]. Masson’s trichrome staining was performed by a ready-to-use kit (Sigma-Aldrich). To calculate the positive index of each staining, three random fields were counted by microscope (BZ-X710; Keyence). Serial sections were used for immunohistochemical staining.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.