PSCs or NAC-PSCs at 90% confluence were transfected with siPPARγ-1(#SI02634275, GS5468, Qiagen, Hilden, Germany) and siPPARγ-2 (#SI00071680) small interfering RNA (siRNA) by electroporation using a Nucleofector System (Lonza, Basel, Switzerland) according to the manufacturer’s recommendations. To verify knockdown specificity, we used a control siRNA (#1027310). Transfected cells were used in subsequent experiments 24–72 h after transfection. Three separate PSC preparations were used.

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