HepG2.2.15 cells transfected by HBV and HepG2 cells were used as subject cells. Cells were cultured in RPMI 1640 medium (Gibco BRL) containing 10% FBS (Gibco BRL). Negative control (NC) siRNA and XIST siRNA were purchased from Thermo Fisher Scientific, Inc. NC and miR-192 mimic were purchased from Gene Pharma (Shanghai, China). Empty vector (p-emptor vector) and lncRNA XIST over-expression vector (p-XIST) were purchased from Shanghai Jima Gene Co., Ltd. (Shanghai, China). These oligonucleotides or plasmids were transfected into HepG2.2.15 or HepG2 cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, US).
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