Prediction of interactions between miR-133a and PTBP1 3ʹuntranslated region (3ʹUTR) was conducted via the StarBase bioinformatics prediction website ( The sequences of PTBP1 3ʹUTR wild-type (wt) or mutant (mt) were constructed by GenScript (Nanjing, Jiangsu, China). Cells were transfected with miR-133a mimic and PTBP1 3ʹUTR (wt) or PTBP1 3ʹUTR (mt) with the help of Lipofectamine 2000 (Invitrogen). After 48 h, luciferase activity was assessed using the dual-luciferase reporter assay system (Promega Corporation, Madison, WI, USA). Firefly luciferase activity was normalized to Renilla luciferase activity.

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