Tumor tissues and the corresponding adjacent tissues were collected from 37 patients with lung cancer in People’s Hospital of Zhengzhou University. All participants did not receive preoperative chemotherapy, radiation, or other anti-cancer therapy before enrollment. Immediately after surgical excision, the tissues were immersed in liquid nitrogen and kept in liquid nitrogen until use. We obtained the written informed consent from each participant before surgery. The Research Ethics Committee of People’s Hospital of Zhengzhou University approved the study protocol. The study was implemented in compliance with the ethical guidelines of the 1975 Declaration of Helsinki.

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