The sequences of BDNF 3ʹUTR containing the binding sites or mutant sites with miR-34b-5p or miR-470-5p were amplified and fused into the psiCHECK2 luciferase vector (Promega, Madison, WI, USA) to generate the WT/MUT-BDNF 3ʹUTR-1 or WT/MUT-BDNF 3ʹUTR-2 vectors, respectively. Then, 20 ng vectors and 50 nM of miRNAs mimic or miR-NC were co-transfected into HEK293T cells (24-well plates, Procell Life Science & Technology). Relative luciferase activities (Firefly/Renilla) were assessed after 48 h using Dual-Glo® Luciferase Assay System (Promega).

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