The oral toxicity test was conceptualized according to OECD guidelines for the Testing of Chemicals (Test No. 425, 2008). Female Sprague-Dawley rats were chosen for testing the toxicity of VOZB. The testing dosages were 55 mg/kg, 175 mg/kg, 550 mg/kg, and 2000 mg/kg according to the guidelines (doses were spaced by a factor of 0.5 on a log dose scale). The different doses of VOZB were uniformly suspended in 1% sodium carboxymethyl cellulose (CMC-Na) solution and orally administered to treated rats after fasting overnight under acute toxicity conditions. Then the mortality of the experimental rats was recorded within a 72 h monitoring period. Our results indicated that neither death nor any abnormal neurobehaviors could be observed with the doses ranging from 55 to 2000 mg/kg during 72 h. Therefore, the 50% lethal dose was greater than 2000 mg/kg.

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