The apoptosis of hippocampal neurons was detected by Annexin V-FITC Apoptosis Detection Kit (Dojindo). Hippocampal neurons were collected and then suspended with 1 × Binging Buffer to obtain the cell suspensions. Subsequently, the cell suspensions were stained with Annexin V-FITC and Propidium iodide for 30 min. The apoptosis rate of cells was examined by a flow cytometry.

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