We adopted the Transwell assay to detect the invasion capability of LoVo and HCT116 cells in vitro. The cells (1 × 105) eluted and re-suspended using the serum-free medium were seeded into Transwell’s parietal chamber which was coated with Matrigel. The culture medium containing 10% fetal bovine serum was placed in the lower chamber. After 24 hours, cells in the lower chamber were removed, fixed, and stained with methanol and 0.1% crystal violet respectively. Five random regions were selected to photograph and count the cells. Said experiment was carried out 3 times.

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