After extracted RNA from the hippocampus tissues and hippocampal neurons using TRIzol reagent (Invitrogen), the RNA was reverse-transcribed into cDNA using the HiScript® II One Step RT-PCR Kit (Vazyme, Nanjing, China). QRT-PCR was performed using SYBR Green (Solarbio, Beijing, China) on PCR system. U6 or GAPDH was utilized as the internal control for miRNA and BNDF, respectively. Relative expression was determined using the 2−ΔΔCT method. The primers used in this research were as below: miR-34b-5p, 5ʹ-CGAGGCAGTGTAATTAGCTGATTGT-3ʹ; miR-470-5p, F, 5ʹ-GTGCGAACCAGTACCTTTCTG-3ʹ, R 5ʹ-GTGCAGGGTCCGAGGT-3ʹ; U6, F 5ʹ-GCTTCGGCAGCACATATACTAAAAT-3ʹ, R 5ʹ-CGCTTCACGAATTTGCGTGTCAT-3ʹ; BDNF, F 5ʹ-AAGGACGCGGACTTGTACAC-3ʹ, R 5ʹ-CGCTAATACTGTCACACACGC-3ʹ; GAPDH, F 5ʹ-CGTCCCGTAGACAAAATGGT-3ʹ, R 5ʹ-TTGATGGCAACAATCTCCAC-3ʹ.

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