The mice were putted in a cylindrical container (water depth of about 30 cm) filled with clean water, and was force to swim. A camera system was used to record the swimming time and immobility time of mice within 5 min. Swimming time referred to the time when the mice upper body or head on the surface of the water and swimming continuously. The immobility time referred to the time when the mice had its head out of the water, floating motionless, or occasionally swimming forelegs to keep the body in balance.

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