For fMRI scans, all preprocessing steps will be performed using DPABI software based on MATLAB. The main analytical methods include the amplitude of low-frequency fluctuation amplitude (ALFF) and seed-based functional connectivity.

After preprocessing the data, the ALFF will be calculated to compare the whole-brain spontaneous activity pattern before and after intervention in each group, as well as between patients with FC and HS following 10-week Tai Chi practices. The different regions obtained at ALFF analysis will be selected as the region of interest (ROI), also called seed, to perform the seed-based functional connectivity analysis and to explore the functional synchronization of ROI and other regions. The thresholds of p < 0.05 with a false discovery rate correlation will be applied to all analyses.

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