2.7. Arsenic Distribution in Wild C. sinensis
This protocol is extracted from research article:
Arsenic Content, Speciation, and Distribution in Wild Cordyceps sinensis
Evid Based Complement Alternat Med, Feb 19, 2021; DOI: 10.1155/2021/6651498

A C. sinensis sample from Zhiduo county was frozen, and sections of 50 μm thickness were made from the stroma, head, thorax, and abdomen (the location of the thin slices of the samples is shown in Figure 1) and pasted on the XRF tape (TF-500) for μ-XRF imaging.

The location of thin slice on C. sinensis.

The distribution of As in C. sinensis samples was analyzed with μ-XRF at the beamline BL15U at the Shanghai Synchrotron Radiation Facility (SSRF, Shanghai, China). The continuous synchrotron X-rags were monochromatized by a Si(111) double-crystal monochromator. A monochromatic X-ray beam with photon energy of 13 keV was used to excite the samples. The cross section of the beam irradiation on the samples was adjusted to about 200 × 200 mm2 with an about 1011 phs/s photon flux. The sample was placed at a 45° angle to the incident X-ray beam, and X-ray fluorescence was detected with a 50 mm2 silicon drift detector (Vortex, USA) oriented at a 90° angle to the incident beam. A light microscope was coupled to a computer for sample viewing. The sample platform was moved by a motorized x-y mapping stage. The As distributions in the sections of different parts of the caterpillar were continuously scanned at a step of 125 μm for both x and y directions. Each spot was irradiated for 5 s.

The X-ray spectra were analyzed by the AXIL program (Canberra Benelux, Belgium), and all the element fluorescence intensities and the Compton scattering intensity were normalized to the collecting time and the changes in I0, which was measured by an upstream ion chamber. The relative quantitative images of metals were obtained using software IGOR Pro 6 (WaveMetrics Inc., USA).

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