2.7.2. Facility Conditions
This protocol is extracted from research article:
Arsenic Content, Speciation, and Distribution in Wild Cordyceps sinensis
Evid Based Complement Alternat Med, Feb 19, 2021; DOI: 10.1155/2021/6651498

The distribution of As in C. sinensis samples was analyzed with μ-XRF at the beamline BL15U at the Shanghai Synchrotron Radiation Facility (SSRF, Shanghai, China). The continuous synchrotron X-rags were monochromatized by a Si(111) double-crystal monochromator. A monochromatic X-ray beam with photon energy of 13 keV was used to excite the samples. The cross section of the beam irradiation on the samples was adjusted to about 200 × 200 mm2 with an about 1011 phs/s photon flux. The sample was placed at a 45° angle to the incident X-ray beam, and X-ray fluorescence was detected with a 50 mm2 silicon drift detector (Vortex, USA) oriented at a 90° angle to the incident beam. A light microscope was coupled to a computer for sample viewing. The sample platform was moved by a motorized x-y mapping stage. The As distributions in the sections of different parts of the caterpillar were continuously scanned at a step of 125 μm for both x and y directions. Each spot was irradiated for 5 s.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.