The amount of ethanol produced by fermentation was estimated by potassium dichromate method [14, 25]. The experiment was performed by dissolving 1 g of potassium dichromate into 27 ml of 98% of sulfuric acid solution. Five clean and dry test tubes were taken containing 00 ml, 0.2 ml, 0.4 ml, 0.6 ml, 0.8 ml, and 1.00 ml of known ethanol concentration, while the sixth test tube was used as blank. Then, 3 ml of prepared chromic acid solution was added to each test tube followed by placing in the water bath at 60°C for 10 minutes. In the end, 1 ml of Rochelle salt solution (40%) was added to stabilize the color. The absorbance was measured at 600 nm using a UV-VIS spectrophotometer.

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