Fermentation media were prepared by placing 100 ml of pretreated and hydrolysed solution into 250 ml flasks. Pretreated and hydrolysed solution was mixed before addition of any microorganism to the prepared samples. The pH of these samples was adjusted between 3.0 and 5.0 before shaking the substrate by using a digital pH meter. The reactor and all the equipment that were used for fermentation purposes were autoclaved at the temperature of 121°C for 15 minutes. After it was cooled in the hood, activated yeast was added into the fermentation medium. Batch types of fermentation were conducted in incubators preset at 180 rpm for 72 hours at 30°C.

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