2.4.1. Sample Ingestion
This protocol is extracted from research article:
Arsenic Content, Speciation, and Distribution in Wild Cordyceps sinensis
Evid Based Complement Alternat Med, Feb 19, 2021; DOI: 10.1155/2021/6651498

An ICP-MS system coupled with the microwave digestion technique was used for sample preparation and detection. C. sinensis powder, 0.25 g, was decomposed using microwave equipment with a mixture of HNO3 (4.0 mL) and H2O2 (2.0 mL). The operating program of the microwave system was as follows: the samples were heated to 120°C from room temperature in 5 min and held for 5 min, then heated to 160°C in 5 min and held for 10 min, and heated to 200°C in 15 min and held for 15 min. During the digestion process the wave power was set to 1800 W. After digestion, the samples were cooled to room temperature. Excess HNO3 was removed by heating the sample solution at 120°C for 20 min. The digestion sample solutions were cooled to room temperature and diluted with ultrapure water up to 50 mL.

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