2.1. Chemicals and Reagents
This protocol is extracted from research article:
Arsenic Content, Speciation, and Distribution in Wild Cordyceps sinensis
Evid Based Complement Alternat Med, Feb 19, 2021; DOI: 10.1155/2021/6651498

Element reference standard solutions of arsenic (As, GBW(E)080117, 1000 μg/ml), arsenite (As(III), GBW08666, 75.7 ± 1.2 μg/g), arsenate (As(V), GBW08667, 17.5 ± 0.4 μg/g), monomethylarsonic acid (MMA, GBW08668, 25.1 ± 0.8 μg/g), dimethylarsinic acid (DMA, GBW08669, 52.9 ± 1.8 μg/g), arsenobetaine (AsB, GBW08670, 38.8 ± 1.1 μg/g), arsenocholine (AsC, GBW08671, 28.0 ± 1.1 μg/g), and standard reference material GBW09588 (Atractylodes macrocephala) were obtained from the National Institute of Metrology (Beijing, China), with their concentrations guaranteed. Working mixture standard solutions of arsenic speciation were prepared daily by diluting the source standard solutions to proper concentrations with pure water. Tune solution and mixed internal standard were provided by PerkinElmer (MA, USA). Na3AsO4, NaAsO2, and As (Cys)3 were also provided by the National Institute of Metrology (Beijing, China).

Nitric acid (HNO3, 68%, ultrapure) and hydrogen peroxide (H2O2, 30%, ultrapure) were purchased from Suzhou Crystal Clear Chemical Co., Ltd. (Suzhou, China). High purity argon (Ar, 99.999 %) was obtained from Jinxin Gas Co., Ltd. (Xining, China). HPLC grade methanol (Merck, Germany), HPLC grade ammonium dihydrogen phosphate, and aqueous ammonia were obtained from ANPEL Laboratory Inc. (Shanghai, China). Ultrapure water (18.2 MΩ) prepared with a Milli-Q system (Millipore, Co., USA) was used for all solution preparations. The glassware and plasticware used in this experiment were soaked in 20% HNO3 solution for 24 h prior to use.

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