2 g of the extractive-free dried sample was placed in a conical flask and 150 mL of NaOH solution (20 g/L) was added to the sample. The mixture was boiled for 3 hours, filtered, and washed with distilled water to remove the NaOH solution. The residue was then dried in an oven for 5 hours at 105°C at a constant weight, cooled at room temperature in a desiccator, and reweighed (W2). The difference between the sample weight before and after treatment was the hemicellulose content [23]. The hemicellulose content sweet sorghum variety was calculated as follows:

where W1 = weight of the sample before drying (2 g); W2 = weight of the sample after drying.

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