The cellular iron concentration level was detected using a Quantichrom Iron Assay kit (cat. no. DIFE-250, BioAssay Systems). PC12 cells were cultured in 96-well plates and transfected with miR-335 mimic, miR-335 inhibitor, miR-335 NC, or siRNA-FTH1, and stimulated with 6-OHDA for 24 h. Following the manufacturer's protocol, 50 µl of standards or samples containing 106 cells were mixed with 200 µl Quantichrom Working Reagent in a 96-well plate and incubated at room temperature overnight. The optical density (OD) was measured at a wavelength of 590 nm using a microplate reader (BioTek Instruments, Inc.).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.