PC12 cells were inoculated in 96-well plates and 3 complex wells. Following induction with various concentrations of 6-OHDA (0, 10, 50 and 250 µM), 20 µl MTS solution (cat. no. G5421; Promega Corporation) mixed with 100 µl RPMI-1640 medium was added to each well at 2 time points (12 and 24 h). Cells were then transferred to a 5% CO2 incubator at 37°C for 1 h. An automated microplate reader was used to detect the absorbance (490 nm) to determine cell proliferation.

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