A total of 24 rats were randomly assigned to 4 groups (6 rats/group) as follows: The control (saline), model (6-OHDA), mimic (6-OHDA + miR-335), or inhibitor (6-OHDA + miR-335 inhibitor). The control group was intracerebrally injected with saline, while the model group, the mimic group, and the inhibitor group received intracerebral injections of 6-OHDA. On post-surgery day 29, the mimic group and the inhibitor group received intracerebral injections of rno-miR-335 mimic (100 pmol/rat; micrON™ miRNA agomir; 5′-UCA AGA GCA AUA ACG AAA AAU GU-3′) or rno-miR-335 inhibitor (200 pmol/rat; micrOFF™ miRNA antagomir; 5′-ACA UUU UUC GUU AUU GCU CUU GA-3′) into the right SNpc: Anteroposterior (A/P)=-4.9 mm, mediolateral (M/L)=−1.9 mm, dorsoventral (D/V)=−7.5 mm). Both products were obtained from Guangzhou RiboBio Co., Ltd.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.