Jag1+/+ and Jag1Ndr/Ndr data were tested for significant differences using multiple tests based on the type of experiment and data distribution. Student´s t-test (Figures 1D, G and and3D,3D, Figure 1—figure supplement 12H, Figure 6—figure supplement 1B–E, H–K). Kolmogorov-Smirnov test (on raw data, graph depicts cumulative sum) (Figures 3C and and4C).4C). Mann-Whitney test (Figure 4—figure supplement 1). Wilcoxon test (Figure 1—figure supplement 5D). Two-way ANOVA (Figures 1H and 5B–D, ,6E,6E, Figure 1—figure supplements 5C and 12I, Figure 6—figure supplement 1F and G) followed by Sidak's multiple comparisons test. Spearman correlation (Figure 1E). A p value below 0.05 was considered statistically significant. The statistical analysis was done in Prism 9 (GraphPad).

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