To evaluate the gap between BD and PV the surface distances were calculated in Matlab for each BD skeleton point by detecting the nearest PV skeleton point and connecting the two points with a line and measuring the non-resin area on this line (zero area in the input binary masks). Surface distance was then calculated using 3D Euclidean distance between the detected non-resin voxel coordinates. The data is represented as cumulative sum of percentage of BD at a given distance from PV (from 0.015 mm to 1.5 mm). The maximum distance between BD and PV for each liver sample was depicted in a separate graph.

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