From each participant, we collected 20 mL of EDTA-anti-coagulated blood that were immediately mixed with an equal volume of 6 M guanidine hydrochloride -0.2M EDTA solution. The target-capture real-time PCR assay used in this study was developed based on the PCR method described by Virreira et al.,7 targeting the kinetoplast T. cruzi mitochondrial minicircle DNA. The DNA extraction was improved with a target-capture step using magnetic beads coated with a T. cruzi-specific 20-mer capture oligonucleotide.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.