NPPB was dissolved in DMSO to form a 0.1 M stock solution. The solution was stored at <−20°C in the dark until use. NPPB was diluted to concentrations of 10-200 µM in serum-free DMEM on the day of the experiment. HLECs were transferred to 96-well plates (100 µl/well) at a density of 0.5×104 cells/well. Cells were incubated for 24 h and treated with 10, 50, 100 and 200 µM of NPPB for 24 h at 37°C. CCK-8 solution (10 µl) was added to each well for 30 min to 1 h at 37°C. The absorbance was expressed as the optical density and was measured at 450 nm using an Infinite M200 Pro microplate reader (Tecan Group, Ltd.).

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