Liver was separated into three regions: R1 (approximately hilum), R2 (approx. intermediate) and R3 (approx. periphery). The optimal region size was calculated as a summary of average PV branch lengths per generation and divided by three (detailed branching generation subdivision Table 3 for PV and Table 4 for BD). Subsequently the branching generations were assigned to a region by matching the summary of branch length per generation to optimal region size. The same region was applied for both PV and BD analysis with the exception of one Jag1Ndr/Ndr sample (#1 or 2714) where the bile duct optimal region size was greater than portal vein.

Regions were assigned in order that total average lengths of each generation within each region yielded an equal size of R1, R2, and R3. Each sub-column represents one animal.

The distribution of BD branches within each region were quantified based on regions defined by the PV system (Table 4). Each sub-column represents one animal.

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