The adult liver was analyzed using a custom-written algorithm and freely available Matlab codes (Version R2017a, The MathWorks Inc, Natick, MA). The algorithm was designed to analyze morphological parameters of the BD and PV systems, and is compatible with the 3D binary masks. Two separate masks of BD and PV system were generated and the analysis was divided in two independent parts. First, the analysis of the entire portal vein and biliary system and second, analysis of the corresponding main branch (=the longest branch) of each system (Figure 6—figure supplement 1A). For detailed analysis and comparison of the whole system versus only the main branch, two algorithms, described by the diagrams in Figure 8F, G, were developed. They differ in the input data and the evaluated parameters. For both algorithms, the first step is to create a 3D skeleton of the input binary mask.

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