Blood from P15 pups was collected from the trunk after decapitation into 1.5 ml tubes. The serum was allowed to clot at room temperature. The blood was centrifuged for 15 min at 17,000 g at room temperature. The serum was stored at −80°C until analyzed. Serum was sent to the Swedish University of Agricultural Sciences for analysis of alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), albumin (Alb), and total bilirubin.

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