Tissue was cleared in methanol/H2O series: 20%, 40%, 60%, 80%, and 100% for 1 hr each at RT. Samples were incubated for 3 hr (with shaking) in 66%DCM (Dichloromethane)/33% methanol at RT and in 100% DCM 15 min 2x (with shaking) to remove traces of methanol. They were incubated in DiBenzyl Ether (DBE) (without shaking).

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