Actin bundles for TEM were prepared by mixing F-actin polymerized from 4 μM G-actin with 1 μM IPP or IPP/Rsu1, and stayed in room temperature for 1 hr. Mixture was dropped to glow discharged, carbon coated copper net (300 mesh), absorbing for 1 min, followed by negatively staining with 1% (w/v) uranium acetate for 30 s. Pictures were captured on TEM (HT7700, HITACHI).

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