Stem cells were differentiated by temporal manipulation of Wnt signaling and metabolic selection. EHTs were assembled in polydimethylsiloxane (PDMS) casting molds (EHT Technologies, Hamburg, Germany) from hPSC-derived cardiomyocytes, fibroblasts, and/or macrophages. EHTs were inoculated with SARS-CoV-2 at least 7 days after tissue seeding.

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