Analytical gel filtration chromatography was carried out on an ÄKTA Pure system (GE Healthcare). Protein samples at indicated concentration were loaded onto a Superdex 200 Increase 10/300 GL column (GE Healthcare) equilibrated with 50 mM Tris-HCl buffer, pH 7.5, containing 100 mM NaCl.

The static light-scattering detector and differential refractive index detector (Wyatt) were coupled to the analytical gel filtration chromatography system. Data were analyzed with ASTRA6 provided by Wyatt.

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