A 33-mer C-terminal Stu2 peptide, with a selenomethionine in place of the natural methionine at position 876 (EESYKRAAAVTSTLKARIEK(Mse)KAKSRREGTTRT), was synthesized at the Tufts University Core facility. The lyophilized peptide was resuspended to a final concentration of 10 mg/mL in a buffer containing 10 mM TRIS pH 7.0, 100 mM NaCl, and 2 mM TCEP.

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