Venous blood samples were taken immediately after hospital admission before PCI from the antecubital vein. The 12-lead electrocardiograms were obtained at baseline and HR was noted. BMI was calculated using the formula weight (kg)/ height2 (m2). The estimated glomerular filtration rate (eGFR) of each patient was calculated using the Chronic Kidney Disease Epidemiology Collaboration equation. Routine blood chemistry, lipid parameters, and troponin-I were measured using standard auto-analyzer equipment. Blood counts were measured using a Sysmex K-1000 (Block Scientific, Bohemia, NY, USA) auto-analyzer. Samples were centrifuged at 3000 rpm for 10 min, and the supernatant and serum separated from the samples. Then they were frozen at -80° C until further analysis. Serum KIM-1 levels (ng/mL) were measured using a commercially available enzyme-linked immunosorbent assay ELISA kit (Human KIM-1 ELISA kit, Elabscience Biotech Co., Ltd, Catalog no: E-EL-H0186, Wuhan, China).4 The KIM-1 kit analysis inter and intra-assay coefficients of variation for the assay was less than 10%, and sensitivity was 0.10 ng/mL.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.