Images were collected with a DeltaVison Elite wide-field microscope system (GE Healthcare) equipped with a scientific CMOS camera, using 60× objective (Olympus; NA = 1.42 PlanApoN) and immersion oil with a refractive index of n = 1.516. A Z-stack was acquired over a 2 µm width with 0.2 µm Z intervals. Images were deconvolved using the DeltaVision algorithm, maximally projected, and analyzed using the Fiji image processing package (ImageJ).

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