The AID system was used essentially as described (Nishimura et al., 2009). Briefly, cells expressed C-terminal fusions of the protein of interest to an auxin responsive protein (IAA7) at the endogenous locus. Cells also expressed TIR1, which is required for auxin-induced degradation. 500 μM IAA (indole-3-acetic acid dissolved in DMSO; Sigma) was added to media to induce degradation of the AID-tagged protein. Auxin was added for 30 min prior to harvesting cells or as is indicated in figure legends.

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