Perfusion of isolated hearts on the Langendorff system and assessment of myocardial injury

The animals were sacrificed by cervical dislocation and hearts were isolated to perfuse on the Langendorff apparatus, using Krebs–Henseleit solution. The inflow of Krebs solution was stopped for 30 min to induce global ischemia. Thereafter, the flow was reinstated for reperfusion for 21 min22. The extent of ischemia-reperfusion injury was assessed by measuring the release of heart-specific CK-MB and cTnT from the heart to the coronary effluent using commercially available diagnostic kits. Moreover, the functional assessment of heart was evaluated by measuring left ventricular developed pressure (LVDP) in the heart using a pressure transducer.

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