One sample was fixed in 4% formalin for 24 h, embedded in paraffin for hematoxylin-eosin (HE) and Picrius Sirius staining.

The vascular density (angiogenesis), dermic appendages (number of hair follicle) were quantified by HE staining under optical microscopy (Nikon eclipse E600, Japan) magnification (× 20, × 40). And by Picrius Sirius staining, the collagen fibers distribution and density (graphic distribution structure) were analyzed. All histological structures mentioned above were analyzed and quantified in 10 fields per slide.

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