At the end of 21 days after the groups’ assignment, the animals were euthanized by an intraperitoneal injection of ketamine hydrochloride (Ketamin, Cristalia, Brazil) 150 mg/kg and xylazine hydrochloride (Rompun, Bayer, Brazil), 10 mg/kg.

Two samples from each lesion were collected. One was prepared for histological analysis and the other was frozen with Nitrogen -80 °C and was performed to analyze IL-10 and MMP-9.

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