At the end of 21 days after the groups’ assignment, the animals were euthanized by an intraperitoneal injection of ketamine hydrochloride (Ketamin, Cristalia, Brazil) 150 mg/kg and xylazine hydrochloride (Rompun, Bayer, Brazil), 10 mg/kg.

Two samples from each lesion were collected. One was prepared for histological analysis and the other was frozen with Nitrogen -80 °C and was performed to analyze IL-10 and MMP-9.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.