All experiments were done in triplicate and the results were presented as mean ± standard error of the mean (SEM). To evaluate the differences between conditions of culture relatively to time and dose of exposure and treatment data were analyzed by two-way analysis of variance (ANOVA) followed by Bonferroni post-test using GraphPad Prism software version 4.00 (GraphPad Software, San Diego, CA, USA). Differences were considered significant at p < 0.05.

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