HCoV-229E and human embryonic lung cell line MRC-5 were obtained from the American Type Culture Collection (CCL-171 and VR-740, respectively). Cells were grown at 37 °C and 5% CO2 in culture media composed of Eagle's minimal essential medium, supplemented with 0.23% (w/v) sodium bicarbonate, 500 μg/mL Penicillin-Streptomycin (ThermoFisher scientific), 1% Glutamax-1, 1% non-essential amino acids, and foetal bovine serum (FBS) 5% (v/v).

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