Organic carbon concentration and isotopic analysis
This protocol is extracted from research article:
Strong local, not global, controls on marine pyrite sulfur isotopes
Sci Adv, Feb 26, 2021; DOI: 10.1126/sciadv.abb7403

Before organic carbon extraction, the carbonate mineral fraction was removed from bulk samples using excess 1.5 N HCl digestion for 48 hours following (70). During digestion, centrifuge tubes were placed in an ultrasonic bath to increase the mechanical separation of clay and calcium carbonates. Total dissolution residues were washed three times with distilled water, centrifuged, and then completely dried at 50°C. The residual powders were homogenized, and before analysis, 30 mg was loaded into tin capsules. Analyses were performed using an elemental analyzer (Thermo Fisher Scientific Flash EA 2000) coupled to an isotope ratio mass spectrometer (Thermo Fisher Scientific Delta V+ EA-IRMS) at Washington University in St. Louis. TOC concentrations were measured using the thermal conductivity detector of Flash EA 2000. Carbon isotope ratios are reported in standard delta notation as per mil deviations from the Pee Dee Belemnite standard, with an analytical error of <0.2‰ (1σ) for organic carbon isotopes.

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