Experiments were performed in replicates and repeated at least twice to ensure reproducibility. Data were entered into an Excel spreadsheet (Microsoft, Redmond, WA, USA) to determine the mean and standard deviation. For further analyses, all data were transferred to R version 3.6.3 (R foundation for statistical computing; Vienna, Austria) software. Unless otherwise stated, Student’s t-test analysis was used to determine the statistical significance among the different conditions tested. A p-value of less than 0.05 was considered significant.

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