Cryptosporidium parvum oocysts were purchased from the National Institute of Agricultural Research (INRA), Nouzilly, France. The isolate was initially sampled from an infected child and maintained in a laboratory by serial passages on calves at the INRA, Nouzilly, France. Several batches of parasites were used in this study. Oocysts in the feces of an experimentally infected calf were concentrated and purified using a commercially available kit (Isolate for ImmunoMagnetic Separation “IMS” of Cryptosporidium oocysts, TCS biosciences, Botolph Claydon, UK) according to the manufacturer’s protocol. Purified oocysts were enumerated in triplicate by a Kova-slide hemocytometer (Hycor, Garden Grove, CA, USA) and were stored at +4 °C in sterile phosphate-buffered saline (PBS) solution until use.

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