Freeze-dried myofibrillar protein was used for the FTIR analysis (Frontier, PerkinElmer, Norwalk, CT, USA). The sample was mixed with KBr (1:100), and the mixture was ground into a uniform powder and pressed into a transparent sheet. Under transmittance mode, all spectra were collected in the wavelength range of 4000 to 400 cm−1 with the resolution of 4 cm−1 at room temperature. Omnic software was used for basic spectrum analysis. The data of the amide I bands were fitted to a Gaussian curve by Peakfit 4.12 software (Seasolve Software, Inc. Framingham, LA, USA). The secondary structure contents of each component were calculated from the peak area.

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