2.2. Low-Field Nuclear Magnetic Resonance (LF-NMR) and Magnetic Resonance Imaging (MRI) Measurements

T2 transverse relaxation data were measured by a MesoQMR23-060H NMR analyzer (Suzhou Niumag Analytical Instrument Co., Suzhou, China). The permanent magnet was kept at 32 °C with a 0.5 T magnetic field strength. A Carr–Purcell–Meiboom–Gill (CPMG) sequence with parameters including 90° pulses, 180° pulses, and π-value of 21.00 μs, 42.00 μs, and 200 μs was used to collect the relaxation decay data. After removing the surface water, the sample was placed in the 60-mm-diameter tube NMR probe and measured under 10,000 echoes with four scan repetitions. The inversion of the CPMG decay data was performed using MultiExp Inv analysis software (Suzhou Niumag Analytical Instrument Co., Suzhou, China) to obtain the T2 distributed curve as well as the relaxation parameters of different proton components.

MRI experiments were performed by the same MesoQMR23-060H NMR analyzer with spin echo (SE) imaging sequence. The scanning parameters of matrix size, field of view, and slice width were 256 mm × 196 mm, 100 mm × 100 mm, and 3 mm, respectively. The echo time (TE) and repetition time (TR) of the T1 weighted image were 40 ms and 660 ms, respectively, while that of the T2 weighted image were 40 ms and 3300 ms. The raw MRI images were processed using OsiriXLite software (v7.0.4, Pixmeo, Geneva, Switzerland) to obtain pseudo-color images and the corresponding intensities.

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