4.5. Quantitative Growth-Based Cell Viability Measurement (Colony Forming Unit Assay)
This protocol is extracted from research article:
Inhibition of Biofilm Formation by the Synergistic Action of EGCG-S and Antibiotics
Antibiotics (Basel), Jan 21, 2021; DOI: 10.3390/antibiotics10020102

Bacterial cultures were incubated at 37 °C for 4 days in 24-well plates. The biofilm attached to the well plate was scrapped and suspended in 100 μL of deionized water. The samples were serially diluted (from 100 to 10−4) and 100 μL of each dilution was spread plated on nutrient agar aseptically. The media plates were incubated overnight at 37 °C. Colony-forming units (CFUs) were recorded and percentage of inhibition was calculated using the following formula. LD50 represents the lethal concentration to 50% of the population.

Additionally, the CFU measurement was used to calculate log10 (fold) reduction using the following equation:

Note: The content above has been extracted from a research article, so it may not display correctly.

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