Reuterin production from L. reuteri DSM 17938 cells was tested by an antagonistic deferred agar spot test [26]. Ten microlitres of sample (free or microencapsulated cells) were spotted on plates of MRS Agar supplemented with 250 mM glycerol and incubated in anaerobic conditions at 37 °C for 24 h. Then, plates were covered with 10 mL of TSB supplemented with 0.75% agar and 0.5% yeast extract and seeded with 2% of an overnight culture of the indicator strain (Escherichia coli 32, Hafnia alvei 53M or Pseudomonas fragi 25P). After the incubation at optimal growth temperature for 24 h, the antimicrobial activity was observed as an inhibition zone of the indicator organism around the sample.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.